Coding

Part:BBa_K4799507

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


GUS

β-glucuronidase， codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2036
    Illegal SpeI site found at 227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 18
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 227
    Illegal NgoMIV site found at 451
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 251
    Illegal BsaI.rc site found at 866
    Illegal SapI.rc site found at 1037


[edit]
Categories
Parameters
None